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10.1098/rstb.2022.0119 137

[56] Cellular aging and autophagy [edit] Pathological aging and metabolic diseases are often characterized by defective mitochondria, the accumulation of misfolded proteins, and cellular senescence

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INS-1 832/3 were maintained in RPMI-1640 at 11 mM d-glucose, supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, 2 mM l-glutamine, 1 mM pyruvate, 50 M -mercaptoethanol, and 1% penicillin/streptomycin 21