Three specimens each of NG, NTS and ARC were incubated for 48 h at 4 C with c-Fos antiserum (Supplementary Table S1), then incubated for 1 h with Alexa Fluor 488- (NTS and ARC) or 594- (NG) conjugated anti-rabbit IgG at room temperature (Supplementary Table S1)
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Additionally, a thorough exploration of immune function and oxidative stress level of kidney-yang deficiency syndrome is warranted to unravel potential pathogenic pathways leading to diarrhea associated with kidney-yang deficiency syndrome
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Primary antibodies targeted FOXO4, Leydig cell markers (StAR, CYP11A1, 3-HSD), spermatogenesis markers (PCNA, PLZF, UCHL1, SCP3), and senescence/apoptosis markers (Ser15-phospho-p53)
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