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Protein concentration was determined using a BCA kit (Thermo Scientific), after which samples were diluted to 1 mg ml 1 in 5% SDS and 50 mM TEAB

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Proper vial sealing, desiccant inclusion, and cold-chain-compatible packaging ensure the compound arrives in the same condition it left the facility

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While the above symptoms can all be triggered by dropping estradiol levels, other health issues may also cause them

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T-TS: Supervision, Writing review and editing, Software

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