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Histopathology Complete histopathology was performed in 24-mo-old female [Old Con ( n = 16), and Old mAb ( n = 16)] and male mice [Old Con ( n = 15), and Old mAb ( n = 17)] following 6 mo of mAb treatment, as well as in female mice at death from the longevity study [Old Con ( n = 30), and Old mAb ( n = 20)]

40 The tubes were cooled on ice and the excess of acid was neutralized using 7.5 M sodium hydroxide solution

Although aging plays a major role in wound healing, it is often neglected in preclinical rodent models and clinical testing, resulting in a limited understanding of the mechanisms by which age slows healing (Gould et al., 2015)