In preferred embodiments amino acid substitutions are made in the amino acid sequence of the Factor VII catalytic triad, defined herein as the regions, which contain the amino acids, which contribute to the Factor VIIa catalytic site
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Subsequently, ATF4 binds to the amino acid response element (AARE) of gene promoter to further promote the transcription of genes involved in amino acid metabolism and stress response, such as SLC7A11, so that cells can cope with amino acid constraints (Kilberg et al., 2009)
Some individuals may experience mild digestive discomfort if taken on an empty stomach
