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They were transiently transfected with truncated GLP-2 receptors attached to LgBiT and G-SmBiT (or G-HiBiT) in a ratio of 1:1 using Lipofectamine 2000 transfection reagent (Invitrogen) and incubated for 24 h

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Neuronal-glial glucose oxidation and glutamatergic-GABAergic function

By improving insulin sensitivity and regulating appetite, these treatments help mitigate hormonal fluctuations that can lead to weight gain and other health issues

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