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Then Tween 20 was added to the mixture gradually, the mixture was homogenized to form nanoemulsions by a high speed homogenizer (Model: 400ELPC, PRO Scientific Inc., 01-02411ELPC homogenizer, USA) at 20,000 rpm for 8 min

The experimental At ASK1 Sh MAX2 Sh HTL7 At SMAX1 complex was superimposed onto the generated SCF scaffold, and the full-length AlphaFold predicted model of At SMAX1 D2 was used