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Samples were then soaked in a 30% sucrose solution for 68 h at 4C, finally included in freezing resin (OCT, Killik, Bio-Optica, Milan, Italy) and cryostat-cut in longitudinal sections (10- m thick)

The findings indicate that phenolic acids (protocatechuic, p -coumaric, chlorogenic and p -hydroxybenzoic acids), flavonoids (hesperidin, quercetin 3- O -rutinoside, naringenin, amentoflavone, cupressuflavone and apigenin) and their derivatives, besides lignans (matairesinol and secoisolariciresinol), coumarins (7-hydroxycoumarin and aesculetin), and the organic compound shikimic acid are the main bioactive molecules isolated from the fruit and leaves of J

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