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the supernatants containing total proteins were collected, and the protein concentration was quantified using a protein assay dye (Bio-Rad)

K63-linked ubiquitination, which typically regulates signaling, DNA repair, and autophagy rather than degradation, is frequently altered in PD, AD, and ALS

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Although its exact mechanisms remain an active area of investigation, DSIP has shown diverse interactions within the central nervous system and endocrine system

These ranges are not always supported by clinical-consensus guidelines or validated across laboratory methods