Membranes were blocked in blocking solution (5% Bovine Serum Albumin, 0.01 M TBS, 0.1% Tween-20) at room temperature for 2 hours, and incubated with primary antibodies (DMP1, 1:1000, Abcam
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Constipationdefined as having fewer than three bowel movements per week, hard or dry stools, or difficulty or pain with passage of stoolis a recognized adverse effect of GLP-1 receptor agonists, though it occurs less frequently than nausea or diarrhea
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N Engl J Med 385(16):14511461 Solomon SD, McMurray JJV, Claggett B, de Boer RA, DeMets D, Hernandez AF et al (2022) Dapagliflozin in heart failure with mildly reduced or preserved ejection fraction