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The cell suspension from each flask was centrifuged at 24 000x g for 30 min at 4 C

[AMA format for Freeman article]: Freeman EW, Sammel MD, Lin H, et al

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Chondrocyte dedifferentiation was majorly characterized as the reduced expression of COL2 and ACAN and enhanced expression of type I collagen and versican ( COL2 expression, but not ACAN ( In addition to dedifferentiation, another consequence of in vitro expansion is replicative senescence, which also significantly influences chondrocyte function

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