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Zhou J, Wu X, Li Z, et al

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According to Horn and colleagues and Milushewa and colleagues, factors such as initial A1C level, adherence to medication, and other underlying health conditions can influence actual A1C reduction

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For amplification, 40 cycles of 95 C for 5 s, and 61 C for 1 s, 72 C for 30 s, then 1 min at 60 C and 10 min at 72 C were performed with Akt forward primer 5-GTGGCAAGATGTGTATGAG-3, Akt reverse primer 5-CTGGCTGAGTAGGAGAAC-3, using Rotor-Gene Q5 plex real-time Rotary analyzer (Corbettlife sciences, USA)