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To explore the effects of the rec-ECM1 protein on liver fibrosis, we built a CCl 4 -induced liver fibrosis mice model based on the methodology outlined in a prior study [20], followed by treatment with two different doses (50 g/Kg or 150 g/Kg) of rec-ECM1 administered biweekly, along with the continuous administration of CCl 4 (Additional File 3 Fig