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Drops (2 L) consisting of a 1:1 ratio of C20A mutant OsDHAR protein (21 mg/mL in 20 mM Tris-HCl, pH 8.0 and 150 mM NaCl) and crystallization solution (100 mM Bis-Tris HCl, pH 6.16.8, 28% (w/v) PEG 3350 and 10 mM CaCl 2 ) were equilibrated against 0.5 mL of the same solution
PubMed, Peng, Qiliu, et al
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This is the same directional change sought with FDA-approved therapies like minoxidil, but achieved through a distinct upstream mechanism
Ab initio calculation of vibrational absorption and circular dichroism spectra using density functional force fields
Regulation of endothelial signaling networks. Nat Rev Immunol
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