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Liquid chromatography-tandem mass spectrometry (LC-MS/MS), which is particularly stable in plasma samples stored at 80C for up to 5 years, can be used to precisely analyze TMAO [5, 85, 86]

Most importantly, this study examined objective markers (e.g

Proteins were then transferred onto a polyvinylidene difluoride (PVDF) membrane using an iBlot2 PVDF stack (Invitrogen) and blocked with 5% bovine serum albumin in tris-buffered saline with 0.1% Tween-20 (TBST) for 1 hour at room temperature

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10.1038/s41575-022-00688-6 50 HuM.ChenY.DengF.ChangB.LuoJ.DongL.et al (2022)