A major limitation of this study was that it relied on parent recall to assess whether advice was given.14 The use of parent recall in research has the advantage of being realistic by assessing whether the parents perceived that they were given advice, but the findings more likely speak to the need for better ways to provide education to parents
Arch Dis Child Fetal Neonatal Ed
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These sources frequently overstate benefits, minimise risks, and fail to account for individual health variables such as cardiovascular status, hormonal baseline, or concurrent medication use
qPCR (20 L) was carried out by using 2 Ultra SYBR Mixture (CWbio, China) in 12 8 well plates in a CFX Connect Real-Time System (Bio-Rad), containing 10 L of SYBR Mixture, 2 L cDNA, 1.6 L of qPCR primers (4 M), and 7.4 L nuclease-free water
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