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Intracellularly, NBT (or DCFH-DA probe) interacts with superoxide, which produces water-insoluble and stable formazan crystals (formazan precipitates after reacting) that can be detected by microscopy for the in situ localization of oxidants, for instance in the sperm pellet
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Comp Biochem Physiol 116B:217221 Carney Almroth B, Albertsson E, Sturve J, Frlin L (2008) Oxidative stress, evident in antioxidant defences and damage products, in rainbow trout caged outside a sewage treatment plant
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