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OBT0030, Bio-Rad, 1:500), COL2 (MAB8887, Chemicon, 1:200) and G6PDH (ab231828, Abcam, 1:500)

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[35] This high requirement for NAD + results from the constant consumption of the coenzyme in reactions such as posttranslational modifications, since the cycling of NAD + between oxidized and reduced forms in redox reactions does not change the overall levels of the coenzyme

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Stromal senescence establishes an immunosuppressive microenvironment that drives tumorigenesis