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Researchers tracking biomarkers (fasting glucose, resting metabolic rate, nitrogen balance) can detect timing-specific metabolic shifts within 710 days

An ion-trap mass spectrometry analyzer was used for tandem mass spectrometry scans in top speed mode, with dynamic exclusion settings of repeat count of 1, repeat duration of 15 s, exclusion duration of 30 s, and a cycle time of 3 s

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Therefore, to investigate whether ROS generation was facilitated by GSTP-silencing, cellular ROS levels were compared between GSTP-silenced and normal conditions (control siRNA transfectant) for 8 cell lines