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Samples were incubated overnight at 4C, rinsed 5 in 1xPBS + 0.1% Triton X-100, then incubated overnight at 4C in secondary antibody + 10% NGS in 1xPBS + 0.1% Triton X-100

This staggered schedule maintains overlapping therapeutic windows (both compounds have multi-day half-lives) while preventing acute gastric paralysis during the first 48 hours post-injection when plasma concentrations peak

And all melasma is exceedingly tough to tackle, unlike dark spots, which can typically be treated with topicals like retinoids and vitamin C

Think of it as an alarm clock for tissue that fell asleep

R., Zhou, H

Lippincott, Williams & Wilkins Trends in Neurosciences