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Smears were prepared each morning and analyzed under a microscope (OLYMPUS, 200x, Japan) using Giemsa staining to determine the predominant cell type and assess the estrus phase 55

doi: 10.1089/thy.2019.0201 69 Van den BergheGWoutersPWeekersFMohanSBaxterRCVeldhuisJDet al

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Results can last for several days to a few weeks, and regular sessions may help maintain long-term skin benefits