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Avoid repeated freezethaw cycles and discard any unused solution according to institutional biosafety guidelines

The two main approaches are subcutaneous injection (under the skin, typically in the abdominal fat pad) and intramuscular injection (into a muscle belly)

To further assess how CI affects transcription, we analysed RNA expression in circadian mutant mice with restored CI function

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These data demonstrate that CaMKK2 is a component of a signalling hub that enables crosstalk between kinase and small G-protein signalling networks that regulate brain function

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