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Biomarkers tested Beyond behavioral testing, the researchers conducted extensive analysis of brain tissue, measuring various crucial markers: [1] Brain-derived neurotrophic factor (BDNF) Key neurotransmitters (acetylcholine, dopamine, GABA) Antioxidant markers (catalase, glutathione) Beta-amyloid levels Cyclic GMP This comprehensive approach allowed the research team to not only observe behavioral outcomes but also understand the underlying neurochemical changes driving these effects

Firstly, the use of BCPB results in clean chromatograms because the derivatization reaction does not yield byproducts and the detector analytical wavelength falls in the UV-region in which the biological matrix is usually transparent