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At the endpoint, 10 L of 3-[4,5-dimethylthiazol-2-yl]-2,5diphenyltetrazolium bromide (MTT agent) was added to the wells, plates were incubated for 4 h at 37 C, followed by 100 L of solubilization buffer

An oxidant/antioxidant imbalance characterized by oxidative stress and low GSH levels is involved in the pathogenesis of idiopathic pulmonary fibrosis, since data show marked GSH deficiency in the lower respiratory tract of those patients (127)

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