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The laccase activity was measured by adding 180 l of reaction mixture (100 mM citratephosphate buffer pH 4.0 and 1 mM ABTS) by using a Multidrop robot (Multidrop Combi, Thermo Scientific)

This enzyme follows first-order kinetics with respect to GSH concentration, so the initial reaction rate varies linearly with GSH molarity 27

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Later, this methodology was adapted and used to study other epithelia of different organs including the GI tract

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