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The concentrations were determined by acquiring chromatograms and then using them to build a calibration curves showing the average peak areas

Another interesting resistin issue was described by Leivo-Korpela et al

Solution: Use a low-and-slow approach

Castelli MS, McGonigle P, Hornby PJ

For any in-vivo animal research conducted in the United Kingdom, full compliance with the Animals (Scientific Procedures) Act 1986 (ASPA) is mandatory, requiring valid Home Office licences and institutional ethical approval

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