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Expanded NK cells were cultured in RPMI (Corning) supplemented with 10% FBS (Genesee Scientific) + 1% of each of the following: penicillin/streptomycin (HyClone), NEAA (Lonza), L-glutamine (Sigma), sodium pyruvate (Lonza), and HEPES (ThermoFisher)

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Its important to rotate the injection sites to avoid skin problems

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